Investigation of the Association of FOLH1 rs61886494 and DISC1 rs12133766 loci in Iranian Schizophrenia Patients

Main Article Content

Azizi Parisa
Azizi Faryal
Nemati Fahimeh


Background: The aim of this study was to identification of polymorphisms of FOLH1 and DISC1 genes in Iranian patients with schizophrenia.

Materials and Methods: In this case-control study, 50 patients with schizophrenia and 50 healthy controls were evaluated. PCR-RFLP and Tetra-ARMS method used for detection of FOLH1 and DISC1 gene respectively in both of patients and control groups.

Results: The frequency of CC, CT, and TT genotypes for FOLH1 gene in rs61886494 locus in schizophrenic patients was 92%, 8%, and 0%, respectively, and in healthy subjects, 94%, 0%, and 6%, respectively. The frequency of DISC gene in GG genotype was higher than that of normal people and frequency of GA genotype was lower than normal subjects. In addition, the genotype AA was identified only in patients.

Conclusion: For FOLH1 gene in rs61886494 locus, the frequency of CC and TT genotypes in patients was 2% and 6% lower in healthy people, while CT genotype in patients was 8% higher in healthy people. Interestingly, TT genotype was not observed in patients and CT genotype in healthy people was not observed. Regarding the DISC1 gene, the results showed that the frequency of homozygous GG and GA homozygote genotypes in the patients was higher in the rs12133766 locus, while the heterozygote GA was high in healthy subjects and was not observed in patients. Therefore, the result of this study in our country can provide suitable method for diagnosis and prevention of schizophrenia patients.

Schizophrenia, polymorphism, DISC1 gene, FOLH1 gene.

Article Details

How to Cite
Parisa, A., Faryal, A., & Fahimeh, N. (2019). Investigation of the Association of FOLH1 rs61886494 and DISC1 rs12133766 loci in Iranian Schizophrenia Patients. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(2), 1-7.
Original Research Article


Vahia VN. Diagnostic and statistical manual of mental disorders 5: A quick glance. Indian Journal of Psychiatry. 2013; 55(3):220-223.

McGrath J, et al. Schizophrenia: a concise overview of incidence, prevalence, and mortality. Epidemiol Rev. 2008;30:67-76.

McGrath JJ. Myths and plain truths about schizophrenia epidemiology--the NAPE lecture 2004. Acta Psychiatr Scand. 2005; 111(1):4-11.

Jablensky A, et al. Schizophrenia: manifestations, incidence and course in different cultures. A World Health Organization ten-country study. Psychol Med Monogr Suppl. 1992;20:1-97.

Kinney DK, et al. Relation of schizophrenia prevalence to latitude, climate, fish consumption, infant mortality, and skin color: A role for prenatal vitamin D deficiency and infections? Schizophrenia Bulletin. 2009;35(3):582-595.

Torrey EF, et al. Seasonal birth patterns of neurological disorders. Neuro-Epidemiology. 2000;19(4):177-85.

McGrath JJ, Welham JL. Season of birth and schizophrenia: A systematic review and meta-analysis of data from the Southern Hemisphere. Schizophr Res. 1999;35(3):237-42.

Bota RG, Munro JS, Sagduyu K. Identification of the schizophrenia prodrome in a hospital-based patient population. Mo Med. 2005;102(2):142-6.

Werbeloff N, Levine SZ, Rabinowitz J. Elaboration on the association between immigration and schizophrenia: A population-based national study disaggregating annual trends, country of origin and sex over 15 years. Soc Psychiatry Psychiatr Epidemiol. 2012; 47(2):303-11.

Bourque F, van der Ven E, Malla A. A meta-analysis of the risk for psychotic disorders among first- and second-generation immigrants. Psychol Med. 2011;41(5):897-91.

Fabisch H, Kroisel PM, Fabisch K. Genetic risk factors in schizophrenia. Fortschr Neurol Psychiatr. 2005;73(Suppl 1):S44-50.

Derlin T, et al. Evaluation of 68Ga-glutamate carboxypeptidase II ligand positron emission tomography for clinical molecular imaging of atherosclerotic plaque neovascularization. Arterioscler Thromb Vasc Biol. 2016;36(11):2213-2219.

Zlitni A, et al. Development of prostate specific membrane antigen targeted ultrasound microbubbles using bioorthogonal chemistry. PLoS One. 2017; 12(5):e0176958.

Lee SS, et al. Prostate-specific membrane antigen-directed nanoparticle targeting for extreme nearfield ablation of prostate cancer cells. Tumour Biol. 2017;39(3): 1010428317695943.

Frigerio B, et al. Full preclinical validation of the 123I-labeled anti-PSMA antibody fragment ScFvD2B for prostate cancer imaging. Oncotarget. 2017;8(7):10919-10930.

Zhou J, et al. NAAG peptidase inhibitors and their potential for diagnosis and therapy. Nat Rev Drug Discov. 2005; 4(12):1015-1026.

Begeron R, Coyle JT. N-Acetyl aspartyl-glutamate, NMDA receptor, and psychosis. Current Medicinal Chemistry. 2012;19(9): 1360-1364.

Thomson PA, et al. Balanced translocation linked to the psychiatric disorder, glutamate, and cortical structure/function. NPJ Schizophrenia. 2016;2:16024.

Jaaro-Peled H, et al. Neurodevelopmental mechanisms of schizophrenia: understanding disturbed postnatal brain maturation through neuregulin-1-ErbB4 and DISC1. Trends Neurosci. 2009;32(9): 485-95.

Patel KR, et al. Schizophrenia: Overview and treatment options. Pharmacy and Therapeutics. 2014;39(9):638-645.

Green MF, Harvey PD. Cognition in schizophrenia: Past, present, and future. Schizophrenia Research. Cognition. 2014; 1(1):e1-e9.

Tai S, Turkington D. The evolution of cognitive behavior therapy for schizophrenia: Current practice and recent developments. Schizophrenia Bulletin. 2009;35(5):865-873.

Hofmann SG, et al. The efficacy of cognitive behavioral therapy: A review of meta-analyses. Cognitive Therapy and Research. 2012;36(5):427-440.

Heidari Keshel S, R.Z.S., Ghasemvand F, Maleki MH. Analysis of polymorphisms in relation to schizophrenia susceptibility. Scientific Journal of Ilam University of Medical Sciences. 2012;20(4):192-199.

Guoqin HU, et al. Association of schizophrenia with the rs821633 polymorphism in the DISC1 gene among Han Chinese. Shanghai Arch Psychiatry. 2015;27(6):348–355.

Kim HJ, Park HJ, Jung KH, Ban JY, Ra J, Kim JW, Park JK, Choe BK, Yim SV, Kwon YK, Chung JH. Association study of polymorphisms between DISC1 and schizophrenia in a Korean population. Neurosci Lett. 2008;430(1):60-3. Epub 2007 Oct 22.

Joshua LR. Genetic variation throughout the Folate Metabolic Pathway Influences Negative Symptom Severity in Schizophrenia. Schizophr Bull. 2013; 39(2):330–338.